Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293 cells transfected with human Rab27a-GFP)
Loading amount
20 µg
Specification
HEK-293 cells transfected with human Rab27a-GFP
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%Milk/1XPBS-Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Additional data
Additional Notes
1. lysate of HEK 293 transfected with empty pCDNA3 vector
2. lysate of HEK 293 cells expressing Rab27a-GFP
Rab27a (25kDa)+GFP(27kDa)=52kDa
2. lysate of HEK 293 cells expressing Rab27a-GFP
Rab27a (25kDa)+GFP(27kDa)=52kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Feb 29 2008