Anti-RAB10 抗体 [4E2] (ab104859)
Key features and details
- Mouse monoclonal [4E2] to RAB10
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-RAB10 antibody [4E2]
RAB10 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4E2] to RAB10 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Purified recombinant fragment of Human RAB10 expressed in E. coli.
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ポジティブ・コントロール
- Hela and NIH/3T3 cell lysates; LOVO cells
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR120151-16 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
4E2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104859の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 23 kDa.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 23 kDa. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280). - Information by UniProt
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参照データベース
- Entrez Gene: 10890 Human
- Entrez Gene: 19325 Mouse
- Omim: 612672 Human
- SwissProt: P61026 Human
- SwissProt: P61027 Mouse
- Unigene: 467960 Human
- Unigene: 378993 Mouse
- Unigene: 486858 Mouse
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別名
- GTP binding protein RAB10 antibody
- Rab10 antibody
- RAB10 member RAS oncogene family antibody
see all
画像
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All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilution
Lane 1 : Hela cell lysate
Lane 2 : NIH/3T3 cell lysate
Predicted band size: 23 kDa -
Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.
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Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (15)
ab104859 は 15 報の論文で使用されています。
- Dai S et al. LncRNA EBLN3P promotes the progression of osteosarcoma through modifying the miR-224-5p/Rab10 signaling axis. Sci Rep 11:1992 (2021). PubMed: 33479458
- Sobu Y et al. Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33653948
- Xiang C et al. Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells. iScience 24:102849 (2021). PubMed: 34381974
- Actis Dato V & Chiabrando GA Activated Alpha-2 Macroglobulin Improves Insulin Response via LRP1 in Lipid-Loaded HL-1 Cardiomyocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34203120
- Kadgien CA et al. Endosomal traffic and glutamate synapse activity are increased in VPS35 D620N mutant knock-in mouse neurons, and resistant to LRRK2 kinase inhibition. Mol Brain 14:143 (2021). PubMed: 34530877