WST-8 Assay Kit (Cell Proliferation) (ab65475)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 4 hr
- Sample type: Adherent cells, Suspension cells
製品の概要
-
製品名
WST-8 Assay Kit (Cell Proliferation)
Cell viability/proliferation キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
全工程の試験時間
4h 00m -
製品の概要
WST-8 Assay Kit ab65475 (previously called WST-1 Assay Kit) is an alternative version of ab65473, our most popular WST-1 Assay Kit (which is also supplied as a ready-to-use reagent ab155902). This kit uses a different WST-8 analog, and also contains a stop solution.
The WST-8 assay provides by far the easiest and most sensitive means for performing a quantitative cell proliferation assay, cell viability assay, or cytotoxicity assay in mammalian cells.
The WST-8 assay protocol is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by microplate reader by measuring the absorbance of the dye solution at 440 nm.
The WST-8 assay protocol is very simple:
- add the WST-8 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
- shake the plate to mix the contents
- analyze the amount of formazan dye produced by measuring the absorbance at 440 nm
This kit contains an optional stop solution to stop the production of formazan dye. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K302 Quick Cell Proliferation colorimetric Assay Kit Plus. K302-2500 is the same size as the 2500 test size of ab65475.
The WST-8 assay is add-and-read, and requires no washing, harvesting, or solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
-
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 500 tests 2500 tests Electrocoupling Solution 1 x 5ml 1 x 25ml Stop Solution IV 1 x 5ml 1 x 25ml WST Reagent I 1 vial 5 vials -
研究分野
-
関連性
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
別名
- Cell Cytotoxicity
- cell tracking
関連製品
-
Assay kits
画像
-
Mitochondrial dehydrogenases checked in various cell concentrations at different incubation times (2h WST for each condition)
-
HL60 cell proliferation measured after 24h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
-
HL60 cell proliferation measured after 48h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
-
HL60 cell proliferation measured after 72h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (20)
ab65475 は 20 報の論文で使用されています。
- Wang M et al. Immunostimulatory activity of soybean hull polysaccharide on macrophages. Exp Ther Med 23:389 (2022). PubMed: 35495602
- Liu Q et al. TOP1 inhibition induces bifurcated JNK/MYC signaling that dictates cancer cell sensitivity. Int J Biol Sci 18:4203-4218 (2022). PubMed: 35844787
- Song J et al. Deficiency of peroxisomal NUDT7 stimulates de novo lipogenesis in hepatocytes. iScience 25:105135 (2022). PubMed: 36185359
- Ramírez-Santos J et al. Understanding the Antilymphoma Activity of Annona macroprophyllata Donn and Its Acyclic Terpenoids: In Vivo, In Vitro, and In Silico Studies. Molecules 27:N/A (2022). PubMed: 36296714
- Pitarresi JR et al. PTHrP Drives Pancreatic Cancer Growth and Metastasis and Reveals a New Therapeutic Vulnerability. Cancer Discov 11:1774-1791 (2021). PubMed: 33589425