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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Overview
Product name
Proteinase K protein (Active)
Protein description
Full length native protein isolated from Tritirachium album.
Properties
Form
Lyophilised:
Reconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Concentration
Concentration information loading...
Additional notes
Warning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.
Research areas
Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
in situ hybridisation
ChIP
SDS-PAGE
Application notes
ChIP: Use at an assay dependent dilution.
ISH: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This enzyme can be used with studies using ab52579, ab46540 and ab46666.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
Protocols
References for Proteinase K protein (Active) (ab51501)
ab51501 has not yet been referenced specifically in any publications.
Publishing research using ab51501? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"