Proteinase K protein (Active) (ab51501)

Product Name 

Proteinase K protein (Active)

Product type 

Proteins and Peptides

Peptide 

Full length native protein isolated from Tritirachium album.

Tested applications 
(see key)

ChIP, ISH, SDS-PAGE

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
ISH: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This enzyme can be used with studies using ab52579, ab46540 and ab46666.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> ChIP Related Products >> ChIP Related Products
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases

Relevance 

Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name.
Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.

Storage buffer 

Preservative: None

Form 

Lyophilised
Reconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Warning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.

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