Application
Western blot
Sample
Mouse Purified protein (heart and spleen)
Loading amount
1 µg
Specification
heart and spleen
Gel Running Conditions
Reduced Denaturing (12,5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 2,5% Milk in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit (Dianova)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 24 crossreactivity w kDa
Positive control
purified 20S proteasome from mouse spleen
Additional data
Additional Notes
the fain band in heart at 24kDa is maybe a crossreactivity of the antibody with the counterpart of LMP2, the constitutive subunit beta1 / delta
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 03 2008