Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mammary epithelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization
No
Specification
Mammary epithelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 594 goat anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with pig and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Katie Elliott
Verified customer
投稿 Jul 15 2016