Anti-Profilin 1 抗体 - Carboxyterminal end (ab50667)
Key features and details
- Rabbit polyclonal to Profilin 1 - Carboxyterminal end
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Profilin 1 antibody - Carboxyterminal end
Profilin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Profilin 1 - Carboxyterminal end -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide:
CYEMASHLRRSQY
conjugated to KLH, corresponding to C terminal amino acids 128-140 of Human Profilin 1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50667の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
|
特記事項 |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
配列類似性
Belongs to the profilin family. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 18643 Mouse
- SwissProt: P62962 Mouse
- Unigene: 2647 Mouse
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別名
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
画像
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Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.
Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.
Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab50667 は 13 報の論文で使用されています。
- Drazic A et al. The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1. J Mol Biol 434:167397 (2022). PubMed: 34896361
- Zi J et al. PFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK. Cells 11:N/A (2022). PubMed: 36291059
- Nejedlá M et al. The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 4:N/A (2021). PubMed: 33184056
- Nekouei M et al. Mutant Profilin1 Aggregation in Amyotrophic Lateral Sclerosis: An in Vivo Biochemical Analysis. Basic Clin Neurosci 12:213-222 (2021). PubMed: 34925718
- Rebowski G et al. Mechanism of actin N-terminal acetylation. Sci Adv 6:eaay8793 (2020). PubMed: 32284999