Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
30 µg
Specification
Breast Cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: peroxidase labeled anti-mouse IgG
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 150 kDa
Additional data
Abcam response
Thank you for submitting this review. We are sorry to hear that it did not work in this instance. We have followed up with this customer in accordance with our AbPromise (www.abcam.com/abpromise).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 04 2013