Application
Western blot
Sample
Streptococcus pneumoniae Cell lysate - whole cell (Streptococcus pneumoniae: serotype 3, strain A66)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Streptococcus pneumoniae: serotype 3, strain A66
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% Milk in TBST
Concentration
1.5 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: m-IgGk BP-HRP (sc-516102)
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 53 kDa Non-specific: none kDa
Positive control
Streptococcus pneumoniae serotype 3 strain A66 cultured for 8 h.
Additional data
Additional Notes
Worked first time. Optomisation of antibody concentration very simple.
Bacterial lysates in 4% SDS with Sigma bacterial protease inhibitor cocktail.
Membrane: nitrocellulose membrane. Semi dry transfer.
Protein ladder NEB P7712S
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 01 2017