Anti-Plzf 抗体 [5B3] (ab104854)
Key features and details
- Mouse monoclonal [5B3] to Plzf
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Plzf antibody [5B3]
Plzf 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5B3] to Plzf -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Purified recombinant fragment of Human Plzf expressed in E. Coli.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035). HeLa cells
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR161367-22 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5B3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104854の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 74 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 74 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
組織特異性
Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Skeletal defects, genital hypoplasia, and mental retardation
A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 9 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7704 Human
- Omim: 176797 Human
- SwissProt: Q05516 Human
- Unigene: 591945 Human
- Unigene: 682144 Human
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別名
- AI467657 antibody
- lu antibody
- PLZF antibody
see all
画像
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Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate
Predicted band size: 74 kDa -
Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (15)
ab104854 は 15 報の論文で使用されています。
- Bashiri Z et al. Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds. Andrologia 54:e14380 (2022). PubMed: 35083770
- Liu R et al. SETDB1 Regulates Porcine Spermatogonial Adhesion and Proliferation through Modulating MMP3/10 Transcription. Cells 11:N/A (2022). PubMed: 35159180
- Mao GP et al. Characterization, isolation, and culture of spermatogonial stem cells in Macaca fascicularis. Asian J Androl 23:240-248 (2021). PubMed: 33533740
- Xu H et al. Derivation and propagation of spermatogonial stem cells from human pluripotent cells. Stem Cell Res Ther 11:408 (2020). PubMed: 32967715
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978