Anti-Plexin B1 抗体 (ab39717)
Key features and details
- Rabbit polyclonal to Plexin B1
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Plexin B1 antibody
Plexin B1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Plexin B1 -
由来種
Rabbit -
特異性
PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved. -
アプリケーション
適用あり: IHC-FoFr, WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.
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ポジティブ・コントロール
- WB: Mouse and rat liver tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39717の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-FoFr |
1/1000.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).
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特記事項 |
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IHC-FoFr
1/1000. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa). |
ターゲット情報
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機能
Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. -
組織特異性
Highly expressed in fetal kidney, and at slightly lower levels in fetal brain, lung and liver. -
配列類似性
Belongs to the plexin family.
Contains 3 IPT/TIG domains.
Contains 1 Sema domain. -
翻訳後修飾
Phosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding.
Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5364 Human
- Entrez Gene: 235611 Mouse
- Entrez Gene: 316009 Rat
- Omim: 601053 Human
- SwissProt: O43157 Human
- SwissProt: Q8CJH3 Mouse
- Unigene: 476209 Human
- Unigene: 53862 Mouse
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別名
- KIAA0407 antibody
- Plexin 5 antibody
- Plexin B1 antibody
see all
画像
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All lanes : Anti-Plexin B1 antibody (ab39717) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab39717 は 3 報の論文で使用されています。
- Gerstmann K et al. A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis. Sci Adv 8:eabo4552 (2022). PubMed: 36399562
- Wu JH et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med 12:e10154 (2020). PubMed: 31943789
- Daviaud N et al. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol 76:882-99 (2016). PubMed: 26579598