Application
Western blot
Sample
Mouse Cell lysate - whole cell (IC21)
Gel Running Conditions
Reduced Denaturing (4-20%, 100V)
Loading amount
10 µg
Treatment
shRNA
Specification
IC21
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Rabbit anti-goat IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 37 kD kDa Non-specific: 75 kD kDa
Positive control
Non-transduced, scramble
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Spencer Goodman
Verified customer
投稿 May 24 2017