Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (porine periodontal ligament cells)
Specification
porine periodontal ligament cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% PBST
Blocking step
1% serum + 0.1% Tween-20 +PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 99.9% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 28 2012