HRP Anti-Phosphotyrosine 抗体 [PY20] (ab16389)
Key features and details
- HRP Mouse monoclonal [PY20] to Phosphotyrosine
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-Phosphotyrosine antibody [PY20]
Phosphotyrosine 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [PY20] to Phosphotyrosine -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
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特記事項
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
PY20 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16389の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/5000.
|
特記事項 |
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WB
1/5000. |
ターゲット情報
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関連性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
画像
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HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab16389 は 17 報の論文で使用されています。
- Carro MLM et al. Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility. Front Cell Dev Biol 9:660165 (2021). PubMed: 34249914
- Hwang JM et al. Novaluron Has Detrimental Effects on Sperm Functions. Int J Environ Res Public Health 19:N/A (2021). PubMed: 35010320
- Hirakata S et al. Genetic Deletion of Socs3 in Smooth Muscle Cells Ameliorates Aortic Dissection in Mice. JACC Basic Transl Sci 5:126-144 (2020). PubMed: 32140621
- Bae JW et al. Vanadium adversely affects sperm motility and capacitation status via protein kinase A activity and tyrosine phosphorylation. Reprod Toxicol 96:195-201 (2020). PubMed: 32659260
- Hayes BH et al. Macrophages show higher levels of engulfment after disruption of cis interactions between CD47 and the checkpoint receptor SIRPa. J Cell Sci 133:N/A (2020). PubMed: 31964705