Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Treatment
UV irradiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/250
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 56 kDa
Positive control
UV irradiated HeLa cells
Negative control
untreated HeLa cells
Additional data
Additional Notes
Chk1 was precipitated with specific antibody. Precipitated Chk1 was then subjected to SDS PAGE and WB using this antibody.
Abcam response
The customer has been contacted and commented that the reason for this rating was that although this antibody worked, it was a very weak antibody with low signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 13 2013