Anti-Phospho - (Ser/Thr) Phe 抗体 (ab17464)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr) Phe
- Suitable for: ELISA, IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Phospho - (Ser/Thr) Phe antibody
Phospho - (Ser/Thr) Phe 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospho - (Ser/Thr) Phe -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IP, WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
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特記事項
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified by Protein A and peptide affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17464の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/500.
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IP |
1/100.
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WB | (5) |
1/1000.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
1/500. |
IP
1/100. |
WB
1/1000. Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
ICC/IF
Use at an assay dependent concentration. |
画像
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To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
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All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
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All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
All lanes : calyculin A treated A431 cells
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (94)
ab17464 は 94 報の論文で使用されています。
- Kanwar P et al. CIPK9 targets VDAC3 and modulates oxidative stress responses in Arabidopsis. Plant J 109:241-260 (2022). PubMed: 34748255
- Yuan Y et al. High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction. EMBO Rep 23:e53466 (2022). PubMed: 34779558
- Ren BX et al. The antibiotic drug trimethoprim suppresses tumour growth and metastasis via targeting Snail. Br J Pharmacol 179:2659-2677 (2022). PubMed: 34855201
- Wang L et al. OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak. J Integr Plant Biol 64:1116-1130 (2022). PubMed: 35293133
- Yu B et al. KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling. Nat Commun 13:2192 (2022). PubMed: 35449131