Application
Western blot
Sample
Mouse Purified protein (recombinant protein)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
2 µg
Treatment
Kinase domain of Obscurin
Specification
recombinant protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in TBST buffer
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: AP-conjugated AffiniPure Goat anti-Rabbit IgG (H+L
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
AP
Negative control
GST were used as a negative control in the lane #3 an #7
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 30, 27, 45 kDa
Positive control
Histone H1 were used as a positive control in the lane #2 and #6
Additional data
Additional Notes
Lane Description:
M = Marker;
Lane 1 = Ctl protein only (ctl for the purified kinase);
Lane 2 = Ctl protein + Histone H1;
Lane 3 = Ctl protein + purified GST;
Lane 4 = Ctl protein + purified GST-tagged substrate;
Lane 5 = purified kinase only;
Lane 6 = purified kinase + Histone H1;
Lane 7 = purified kinase + purified GST;
Lane 8 = purified kinase + purified GST-tagged substrate;
Abcam response
Thank you so much for testing this antibody in WB.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application. The customer received a discount on a future antibody purchase for testing this product.
We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Li Wang
Verified customer
投稿 Mar 25 2016