Application
Western blot
Sample
Human Recombinant protein (Estrogen Receptor peptides)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
2 µg
Specification
Estrogen Receptor peptides
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: NA9340V, GE Healthcare
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Lane 2 - 11-amino acid peptide containing non-phosphorylated Serine at position 6.
Exposure
30 second(s)
Bands
Specific: 1.2 kDa Non-specific: 1.2 kDa
Positive control
Lane 1 - 11-amino acid peptide containing phosphorylated Serine at position 6.
Additional data
Additional Notes
This antibody recognized the negative control as well as (if not better than) the positive control in WB.
Abcam response
Thank you for submitting your feedback. I am sorry that the antibody did not work well for you and have set up a refund as per our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Emiliya Storman
Verified customer
投稿 Dec 07 2017