Anti-phospholipase A2 抗体 (ab23705)
Key features and details
- Rabbit polyclonal to Phospholipase A2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-phospholipase A2 antibody
Phospholipase A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholipase A2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
非交差種: Rabbit, Cow, Pig -
免疫原
Human recombinant sPLA2 (Type IIa).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23705の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 14 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 14 kDa. |
ターゲット情報
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機能
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
配列類似性
Belongs to the phospholipase A2 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5320 Human
- Omim: 172411 Human
- SwissProt: P14555 Human
- Unigene: 466804 Human
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別名
- EC 3.1.1.4 antibody
- GIIC sPLA2 antibody
- Group IIA phospholipase A2 antibody
see all
画像
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Western blot detection of human phospholipase A2 (25µg)
ab23705 at 1/1000 western blot detection of 0.25 ug of human phospolipase A2.
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IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (16)
ab23705 は 16 報の論文で使用されています。
- Falidas E et al. Secretory Phospholipase A2 Digital Expression Analysis in Colon Adenocarcinoma. In Vivo 36:738-742 (2022). PubMed: 35241529
- Wang Y et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
- Wei Y et al. Phospholipase A2 inhibitor-loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression. Sci Adv 7:N/A (2021). PubMed: 33827816
- Mohammadtursun N et al. Loki zupa alleviates inflammatory and fibrotic responses in cigarette smoke induced rat model of chronic obstructive pulmonary disease. Chin Med 15:92 (2020). PubMed: 32874197
- Papadopoulos S et al. Secretory Phospholipase A2-IIA Protein and mRNA Pools in Extracellular Vesicles of Bronchoalveolar Lavage Fluid from Patients with Early Acute Respiratory Distress Syndrome: A New Perception in the Dissemination of Inflammation? Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 33238426