Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate (pH 6.0)
Permeabilization
No
Specification
skeletal muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal Goat serum in PBS
Dilution
1/2000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit Ig G (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Sample: estrogen-deplete fetal skeletal muscle
After secondary antibody incubation, sample was treated with ABC solution for 45 min, followed by DAB solution for 5 min and counterstained with Hematoxylin (1:2 dilution in dH2O).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Soon-Ok Kim
Verified customer
投稿 Oct 02 2017