Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
Permeabilization
No
Other product details
Dilution
1/2000
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit anti-mouse, Leica Refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
many antigen retrieval regimes tested EDTA gave best signal/background.
Endothelial and basement membrane marker
Endothelial and basement membrane marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 12 2011