Application
Western blot
Sample
Rat Cell lysate - whole cell (rat benign mammary cell line)
Loading amount
20 µg
Specification
rat benign mammary cell line
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T + 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Polyclonal Swine anti Rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
Additional data
Additional Notes
6X 5 min wash with TBS-T after primary and secondary antibody incubation. Very specific band seen at 65KDa, no unspecific bands.
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投稿 Jan 04 2013