Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Mardin-Darby bovine kidney cells)
Specification
Mardin-Darby bovine kidney cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton-X-100 (20 min)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA-PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (invitrogen)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Untreated Mardin-Darby bovine kidney cells fixed with paraformaldehyde-PBS (4%) grown on uncoated glass coverslips. x100 objective (oil immersion). Nuclei stained with DAPI.
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投稿 Oct 16 2012