Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (PH6)
Permeabilization
No
Specification
kidney
Blocking step
x0909 (DAKO blocking product) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Histofine Simple Stain Max PO (Nichirei Bioscience
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Kidney section at recovery phase from acute kidney injury. Nephrotoxin was administrated to SD rat intravenously. Fourteen days after administration, serum creatinine returned to normal level, then kidneys were harvested, and fixed with 4%PFA.
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投稿 May 29 2017