Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Human Cell (differentiated iPSC)
Specification
differentiated iPSC
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% BSA 0.2% Triton X in PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes Donkey Anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/800
Additional data
Abcam response
Thank you for submitting the feedback. We are pleased to see this antibody has worked well in ICC-IF which is an application the antibody has not been tested in before.
We would be pleased to receive feedback and Abreviews from other customers who have used the antibody in this application to hep verify how well it works in ICC-IF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 26 2015