Anti-PURA 抗体 (ab125200)
Key features and details
- Rabbit polyclonal to PURA
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PURA antibody
PURA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PURA -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human PURA (NP_005850.1).
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab125200 was affinity purified using an epitope specific to PURA immobilized on a solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. -
配列類似性
Belongs to the PUR DNA-binding protein family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5813 Human
- Entrez Gene: 19290 Mouse
- Entrez Gene: 307498 Rat
- Omim: 600473 Human
- SwissProt: Q00577 Human
- SwissProt: P42669 Mouse
- SwissProt: P86252 Rat
- Unigene: 443121 Human
see all -
別名
- PUR ALPHA antibody
- PUR1 antibody
- purA antibody
see all
画像
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Detection of PURA by Western Blot of Immunprecipitate. Lane 1; an antibody to PURA at 1µg/ml staining PURA in HeLa whole cell lysate immunoprecipitated using ab125200 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 10 seconds.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab125200 は 3 報の論文で使用されています。
- Tian L et al. Forming cytoplasmic stress granules PURα suppresses mRNA translation initiation of IGFBP3 to promote esophageal squamous cell carcinoma progression. Oncogene 41:4336-4348 (2022). PubMed: 35945453
- Sun Y et al. PURa Promotes the Transcriptional Activation of PCK2 in Oesophageal Squamous Cell Carcinoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 33142842
- Chang KC et al. MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression. Nat Commun 11:6438 (2020). PubMed: 33353933