Application
Western blot
Sample
Mouse Cell lysate - whole cell (brain)
Loading amount
25 µg
Specification
brain
Treatment
saline 0.9%-6 hrs, ethanol 20%-6 hrs
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 23 kDa Non-specific: 27, 18 kDa
Positive control
Human SH-SY5Y cells treated with Cytosine Arabinoside.
Negative control
Puma knockout mouse brain lysates treated with saline or ethanol.
Additional data
PubMed ID
19535997: View
Additional Notes
No specific bands observed at the right molecular weight even after longer exposures in either wild-type or knockout brain lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Arindam Ghosh
Verified customer
投稿 Mar 08 2011