Anti-PTP1B 抗体 (ab88481)
Key features and details
- Rabbit polyclonal to PTP1B
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-PTP1B antibody
PTP1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PTP1B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide, corresponding to amino acids 146-157 of Human PTP1B
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88481の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
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機能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 19246 Mouse
- Entrez Gene: 24697 Rat
- SwissProt: P35821 Mouse
- SwissProt: P20417 Rat
- Unigene: 277916 Mouse
- Unigene: 11317 Rat
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別名
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
画像
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All lanes : Anti-PTP1B antibody (ab88481) at 1/1000 dilution (in 5% non-fat milk, PBS, 0.04% Tween20
for 1hour at room temperature)
Lane 1 : Mouse SYF cSrc-transformed cells,
untreated
Lane 2 : Mouse SYF cSrc-transformed cells treated with
pervanadate (1 mM for 30 minutes)
Predicted band size: 50 kDa
Observed band size: 50 kDa
The 50kDa band observed can be specifically blocked using the immunogenic peptide, corresponding to PTP1B amino acids 146-157.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab88481 は 3 報の論文で使用されています。
- Papaioannou A et al. Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs. Life Sci Alliance 5:N/A (2022). PubMed: 35193953
- Geng D et al. Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels. Commun Biol 3:306 (2020). PubMed: 32533049
- Haidar M et al. miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence. PLoS Pathog 14:e1006942 (2018). PubMed: 29570727