Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell lines)
Loading amount
20 µg
Specification
Breast cancer cell lines
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
30 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 54 kDa
Additional data
Additional Notes
Lane = MCF-7 breast cancer cell line.
The cell line has been probed for both PTEN (top band) and Beta-actin (bottom band).
The cell line has been probed for both PTEN (top band) and Beta-actin (bottom band).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 15 2010