Anti-PSMD14 抗体 [EPR4257] (ab109123)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4257] to PSMD14
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PSMD14 antibody [EPR4257]
PSMD14 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4257] to PSMD14 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4257 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109123の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C allow to cool for 10-20 min). |
|
ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear. -
組織特異性
Widely expressed. Highest levels in heart and skeletal muscle. -
配列類似性
Belongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 10213 Human
- Entrez Gene: 59029 Mouse
- Entrez Gene: 311078 Rat
- Omim: 607173 Human
- SwissProt: O00487 Human
- SwissProt: O35593 Mouse
- Unigene: 567410 Human
- Unigene: 218198 Mouse
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別名
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
see all
画像
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All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution
Lane 1 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Namalwa (Burkitt's lymphoma cell line) whole cell lysates
Lane 5 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109123 は 4 報の論文で使用されています。
- Bashore C et al. Targeted degradation via direct 26S proteasome recruitment. Nat Chem Biol 19:55-63 (2023). PubMed: 36577875
- Sun T et al. Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2. Mol Oncol 15:3639-3658 (2021). PubMed: 34382324
- Lei J et al. The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer. Ann Transl Med 9:1019 (2021). PubMed: 34277819
- Sakai W et al. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair. Sci Rep 10:19704 (2020). PubMed: 33184426