Application
Western blot
Sample
Mouse Purified protein (Liver and brain)
Gel Running Conditions
Reduced Denaturing (12,5% SDS gel)
Loading amount
2 µg
Specification
Liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% BSA in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: ca. 23 kDa
Positive control
human 20S
Additional data
Additional Notes
Lane 1: mouse liver
Lane 2: rat liver
Lane 3: human erythrocyte
Lane 4: mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 08 2008