Anti-PRUNE 抗体 (ab88613)
Key features and details
- Mouse polyclonal to PRUNE
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRUNE antibody
PRUNE 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PRUNE -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein within Human PRUNE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: Raw 264.7 cell lysate, NIH/3T3 cell lysate, and PRUNE transfected 293T cell lysate ICC/IF: HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88613の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. -
組織特異性
Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. -
配列類似性
Belongs to the PPase class C family. Prune subfamily. -
細胞内局在
Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed. - Information by UniProt
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参照データベース
- Entrez Gene: 58497 Human
- Entrez Gene: 229589 Mouse
- SwissProt: Q86TP1 Human
- SwissProt: Q8BIW1 Mouse
- Unigene: 728223 Human
- Unigene: 14155 Mouse
- Unigene: 279287 Mouse
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別名
- DRES-17 antibody
- DRES17 antibody
- Drosophila-related expressed sequence 17 antibody
see all
画像
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Anti-PRUNE antibody (ab88613) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PRUNE antibody (ab88613) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PRUNE antibody (ab88613) at 1 µg/ml
Lane 1 : PRUNE transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab88613, at 10 µg/ml, staining PRUNE in HeLa cell by Immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab88613 は 2 報の論文で使用されています。
- Santoro D et al. Evaluation of the expression and distribution of Prune-1 in the skin of healthy dogs. Vet Dermatol 30:446-447 (2019). PubMed: 31286578
- Zollo M et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). IF . PubMed: 28334956