Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (egg extract with sperm nuclei)
Permeabilization
Yes - methanol
Specification
egg extract with sperm nuclei
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 3% BSA
Dilution
1/300
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen goat anti-rabbit Alexa-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
sDMR - symmetric dimethylarginine (present on e.g. Sm proteins) Prp8 - homolog of human PRPF8
Abcam response
Thank you for your valuable feedback. There is nuclear stainging in IF on Xenopus laevis egg extract using this antibody to PRPF8, but no clear nuclear speckles are observed suggesting that the antibody under these conditions is not suitable for IF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 27 2011