Anti-PRP6/ANT-1 抗体 (ab99292)
Key features and details
- Rabbit polyclonal to PRP6/ANT-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRP6/ANT-1 antibody -
製品の詳細
Rabbit polyclonal to PRP6/ANT-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human PRP6/ANT-1 aa 1-100. NP_036601.2
Database link: O94906 -
ポジティブ・コントロール
- HeLa, 293T and NIH3T3 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99292の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 107 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation. -
配列類似性
Contains 9 HAT repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 534228 Cow
- Entrez Gene: 24148 Human
- Entrez Gene: 68879 Mouse
- Entrez Gene: 366276 Rat
- Omim: 613979 Human
- SwissProt: Q2KJJ0 Cow
- SwissProt: O94906 Human
- SwissProt: Q91YR7 Mouse
see all -
別名
- Androgen receptor N terminal domain transactivating protein 1 antibody
- ANT 1 antibody
- ANT1 antibody
see all
画像
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All lanes : Anti-PRP6/ANT-1 antibody (ab99292) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 107 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6/ANT-1 with ab99292 at 1/200 (1µg/ml). Detection: DAB.
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Lane 1 : Detection of Human PRP6/ANT-1 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3µg/ml ab99292. Lane 2: Control IgG
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab99292 は 3 報の論文で使用されています。
- Boudreault S et al. Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components. Nucleic Acids Res 50:5263-5281 (2022). PubMed: 35489070
- Cloutier P et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . PubMed: 28561026
- Liu YC et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . PubMed: 24968230