Application
Western blot
Sample
Human Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit Ig(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
24 hour(s), 0 minute(s) and 0 second(s)
Additional data
Abcam response
The samples for this WB were reduced and denatured but run on a precast native gel. The negative results may be indicative of this discrepancy. We do not recommend running reduced/denatured samples on gels made for Native WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 17 2017