Application
Western blot
Sample
Human Cell lysate - whole cell (u2os)
Loading amount
80 µg
Specification
u2os
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
0.5 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 73 kDa
Additional data
Additional Notes
All lanes: PRMT5 antibody (ab31751)
Lane 1 : U2OS Whole Cell Lysate
Predicted band size : 75 kDa
Lane 1 : U2OS Whole Cell Lysate
Predicted band size : 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 10 2011