Anti-PRMT1 抗体 [EPR3292] (ab92299)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3292] to PRMT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PRMT1 antibody [EPR3292]
PRMT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3292] to PRMT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human PRMT1 aa 300-400. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: A549 cell lysate, HeLa cell lysate, NIH/3T3 lysate. IHC-P: human breast cancer tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3292 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
IHC-P |
特記事項 |
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WB: 1/5000 - 1/10000. Predicted molecular weight: 42 kDa.
Is unsuitable for Flow Cyt, ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the protein arginine N-methyltransferase family. -
細胞内局在
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 3276 Human
- Entrez Gene: 15469 Mouse
- Omim: 602950 Human
- SwissProt: Q99873 Human
- SwissProt: Q9JIF0 Mouse
- Unigene: 20521 Human
- Unigene: 27545 Mouse
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別名
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
see all
画像
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All lanes : Anti-PRMT1 antibody [EPR3292] (ab92299) at 1/5000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using ab92299 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab92299 は 4 報の論文で使用されています。
- Filipovic J et al. PRMT1 expression in renal cell tumors- application in differential diagnosis and prognostic relevance. Diagn Pathol 14:120 (2019). PubMed: 31655611
- Sato A et al. Gestational changes in PRMT1 expression of murine placentas. Placenta 65:47-54 (2018). PubMed: 29908641
- Murata K et al. PRMT1 Deficiency in Mouse Juvenile Heart Induces Dilated Cardiomyopathy and Reveals Cryptic Alternative Splicing Products. iScience 8:200-213 (2018). PubMed: 30321814
- Sivakumaran H et al. Overexpression of PRMT6 does not suppress HIV-1 Tat transactivation in cells naturally lacking PRMT6. Virol J 10:207 (2013). WB ; Human . PubMed: 23800116