Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, embryonic day 15.5
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
We were unable to detect a clear signal above background, but we have only tried E15.5 and P6 sections. I have rated this antibody as inconclusive, because we are not sure if PRDM1/Blimp1 should be expressed as late as E15.5. Phalloidin (red), which stains F-actin was used as a counterstain.
Abcam response
Thank you for sharing this data with us. ab13700 has not previously been tested on mouse or in IHC-Fr. We would welcome feedback from other customers who are using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Apr 28 2016