Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Pig Cell (Gonad day 25 fetus)
Specification
Gonad day 25 fetus
Permeabilization
Yes - citrate antigen retrival
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2 % BSA
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Jackson immunoresearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/500
Additional data
Additional Notes
This antibody gave too much background and was not specific to germ cells in the gonad compared to a similar product from a different company.
Abcam response
We appreciate the valuable data provided in this Abreview. To our knowledge ab13700 has not been tested in ICC/IF or with pig samples. The antibody is predicted to detect the target protein in pig due to high homology with the immunogen. Regrettably the results have not been successful in this case. We would welcome feedback from other researchers using ab13700 in ICC/IF or with pig samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ramiro Alberio
Verified customer
投稿 Jul 23 2014