Anti-PRAME 抗体 (ab89097)
Key features and details
- Mouse polyclonal to PRAME
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PRAME antibody
PRAME 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PRAME -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PRAME. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035), PRAME transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. -
組織特異性
Expressed in testis. Detected in samples of kidney, brain and skin. -
配列類似性
Belongs to the PRAME family.
Contains 4 LRR (leucine-rich) repeats. -
細胞内局在
Nucleus. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23532 Human
- Omim: 606021 Human
- SwissProt: P78395 Human
- Unigene: 30743 Human
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別名
- 4930534P07Rik antibody
- Cancer/testis antigen 130 antibody
- CT130 antibody
see all
画像
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Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PRAME antibody (ab89097) at 1 µg/ml
Lane 1 : PRAME transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab89097 は 2 報の論文で使用されています。
- Sivaccumar JP et al. Development of a New Highly Selective Monoclonal Antibody against Preferentially Expressed Antigen in Melanoma (PRAME) and Identification of the Target Epitope by Bio-Layer Interferometry. Int J Mol Sci 22:N/A (2021). PubMed: 33804612
- Nettersheim D et al. The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. Br J Cancer 115:454-64 (2016). PubMed: 27441500