Anti-PPP2R5A 抗体 (ab89621)
Key features and details
- Mouse polyclonal to PPP2R5A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPP2R5A antibody
PPP2R5A 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PPP2R5A -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PPP2R5A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human placenta tissue lysate Jurkat cell lysate Transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89621の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. |
ターゲット情報
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機能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
組織特異性
Widely expressed with the highest expression in heart and skeletal muscle. -
配列類似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 5525 Human
- Omim: 601643 Human
- SwissProt: Q15172 Human
- Unigene: 497684 Human
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別名
- 2A5A_HUMAN antibody
- B56A antibody
- MGC131915 antibody
see all
画像
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Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Predicted band size: 56 kDa -
Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 56 kDa -
All lanes : Anti-PPP2R5A antibody (ab89621) at 1 µg/ml
Lane 1 : PPP2R5A transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 56 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (4)
ab89621 は 4 報の論文で使用されています。
- Vangala D et al. Secondary resistance to anti-EGFR therapy by transcriptional reprogramming in patient-derived colorectal cancer models. Genome Med 13:116 (2021). PubMed: 34271981
- Qiu Z et al. A Genome-Wide Pooled shRNA Screen Identifies PPP2R2A as a Predictive Biomarker for the Response to ATR and CHK1 Inhibitors. Cancer Res 80:3305-3318 (2020). PubMed: 32522823
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223
- Porter IM et al. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun 4:2677 (2013). WB ; Human . PubMed: 24157919