Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
Loading amount
25 µg
Specification
Mouse Embryonic Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti-mouse IgG, HRP linked
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: very weak, 43 kDa
Additional data
Additional Notes
It is a very good antibody but need to use with higher concentration 1:100 and ECL+.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 27 2009