Anti-POMC 抗体 (ab32893)
Key features and details
- Goat polyclonal to POMC
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ELISA
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-POMC antibody
POMC 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to POMC -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra), ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human POMC aa 256-267 (C terminal) (Cysteine residue).
Sequence:C-NAIIKNAYKKGE
Database link: P01189 -
ポジティブ・コントロール
- IHC-P: Human pituitary gland; Flow Cyt (intra): A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32893の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only. |
|
IHC-P | (2) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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ELISA |
1/128000.
Peptide ELISA |
特記事項 |
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WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). A 1 hour primary incubation is recommended for this product. This product appears to react with the full length precursor protein only. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ELISA
1/128000. Peptide ELISA |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 5443 Human
- Omim: 176830 Human
- SwissProt: P01189 Human
- Unigene: 1897 Human
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別名
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
画像
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Flow cytometry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells (blue line) labeling POMC with ab32893 at 10 µg/mL. Cells permeabilized with 0.5% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab32893 は 26 報の論文で使用されています。
- Xia L et al. Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells. J Cell Mol Med 25:2404-2417 (2021). PubMed: 33491272
- Lee EY et al. The local hypothalamic-pituitary-adrenal axis in cultured human dermal papilla cells. BMC Mol Cell Biol 21:42 (2020). PubMed: 32522165
- Wu Z et al. Deletion of liver kinase B1 in POMC neurons predisposes to diet-induced obesity. Life Sci 258:118204 (2020). PubMed: 32763296
- Alkan I et al. Is vagal stimulation or inhibition benefit on the regulation of the stomach brain axis in obesity? Nutr Neurosci N/A:1-13 (2020). PubMed: 33034260
- Zhang S et al. Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development. Nat Commun 11:5275 (2020). PubMed: 33077725