Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12 %)
Sample
Human Cell lysate - whole cell (neuroblastoma)
Specification
neuroblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 16°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: 4% milk
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-rabbit IgG, HRP-linked antibody #7074 ce
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 70 kDa
Exposure
30 second(s)
Additional data
Additional Notes
WB type
membrane : PVDF membrane
lysis buffer : Laemmli sample buffer ( Biorad cat. 161-0737)
Lane1 : SH-SY5Y
Lane 2 : HeLa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 15 2014