Anti-PML Protein 抗体 (ab67761)
Key features and details
- Rabbit polyclonal to PML Protein
- Suitable for: IHC-P, IHC-FoFr, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PML Protein antibody
PML Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PML Protein -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-FoFr, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide derived from internal domain (400-500) of mouse PML protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67761の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 23518714
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WB |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 23518714 |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. -
関連疾患
Note=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon. -
配列類似性
Contains 2 B box-type zinc fingers.
Contains 1 RING-type zinc finger. -
ドメイン
Interacts with PKM2 via its coiled-coil domain.
Binds arsenic via the RING-type zinc finger. -
翻訳後修飾
Ubiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
Phosphorylated in response to DNA damage, probably by ATR.
Acetylation may promote sumoylation and enhance induction of apoptosis. -
細胞内局在
Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein. - Information by UniProt
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参照データベース
- Entrez Gene: 18854 Mouse
- SwissProt: Q60953 Mouse
- Unigene: 392123 Mouse
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別名
- Acure promyelocytic leukemia, inducer of antibody
- MYL antibody
- Pml antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab67761 は 3 報の論文で使用されています。
- Jiao X et al. Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool. Stem Cell Reports 12:135-151 (2019). PubMed: 30554919
- Chort A et al. Interferon beta induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice. Brain 136:1732-45 (2013). IHC-Fr, WB, IHC-P ; Mouse, Human . PubMed: 23518714
- Erickson KD et al. Virion assembly factories in the nucleus of polyomavirus-infected cells. PLoS Pathog 8:e1002630 (2012). IHC-FoFr . PubMed: 22496654