Anti-PLGF 抗体 - N-terminal (ab9542)
Key features and details
- Rabbit polyclonal to PLGF - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-PLGF antibody - N-terminal
PLGF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PLGF - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
Database link: P49763 -
特記事項
According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9542の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 - 4 µg/ml.
It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 4 µg/ml. It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
組織特異性
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
ドメイン
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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参照データベース
- Entrez Gene: 5228 Human
- Omim: 601121 Human
- SwissProt: P49763 Human
- Unigene: 252820 Human
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別名
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
see all
画像
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Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
PlGF antibody shows no cross-reactivity to VEGF165. -
ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (21)
ab9542 は 21 報の論文で使用されています。
- Kang CM et al. Distinctive cytokine profiles of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. J Dent Sci 17:276-283 (2022). PubMed: 35028048
- Carnevale L et al. Celiac Vagus Nerve Stimulation Recapitulates Angiotensin II-Induced Splenic Noradrenergic Activation, Driving Egress of CD8 Effector Cells. Cell Rep 33:108494 (2020). PubMed: 33326772
- Goveia J et al. An Integrated Gene Expression Landscape Profiling Approach to Identify Lung Tumor Endothelial Cell Heterogeneity and Angiogenic Candidates. Cancer Cell 37:21-36.e13 (2020). PubMed: 31935371
- Jiao W et al. Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture. Sci Rep 9:15339 (2019). PubMed: 31653890
- Balser C et al. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina. J Neuroinflammation 16:26 (2019). PubMed: 30732627