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RabMAb

Anti-PKR (phospho T451) 抗体 [EPR2152Y] (ab81303)

製品の概要

  • 製品名
    Anti-PKR (phospho T451) antibody [EPR2152Y]
    PKR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2152Y] to PKR (phospho T451)
  • 特異性
    ab81303 only detects PKR phosphorylated on Threonine 451.
  • アプリケーション
    適用あり: Dot blot, WBmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr451 of Human PKR.

  • ポジティブ・コントロール
    • WB: HeLa cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR2152Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000.
WB Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Double stranded RNA activated protein kinase; antibody
      • E2AK2_HUMAN antibody
      • eIF-2A protein kinase 2 antibody
      • EIF2AK1 antibody
      • EIF2AK2 antibody
      • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
      • HGNC:9437 antibody
      • Interferon induced double stranded RNA activated protein kinase antibody
      • Interferon inducible elF2 alpha kinase antibody
      • Interferon inducible RNA dependent protein kinase antibody
      • Interferon-induced, double-stranded RNA-activated protein kinase antibody
      • Interferon-inducible RNA-dependent protein kinase antibody
      • MGC126524 antibody
      • P1/eIF-2A protein kinase antibody
      • P1/eIF2A protein kinase antibody
      • p68 kinase antibody
      • PKR antibody
      • PPP1R83 antibody
      • PRKR antibody
      • Protein kinase interferon inducible double stranded RNA dependent antibody
      • Protein kinase RNA activated antibody
      • Protein kinase RNA-activated antibody
      • Protein phosphatase 1 regulatory subunit 83 antibody
      • Serine/threonine protein kinase TIK antibody
      • Tyrosine protein kinase EIF2AK2 antibody
      see all

    画像

    • All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma) treated with Interferon-a and Calyculin A whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 62 kDa

      Purified format.

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution (undiluted)

      Lane 1 : HeLa cell lysate un-treated
      Lane 2 : HeLa cell lysate treated with IFN-alpha

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    • Dot blot analysis of PKR (pT451) phospho peptide (Lane 1), PKR non-phospho peptide (Lane 2), labeling PKR (phospho T451) with ab81303 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes

    プロトコール

    参考文献

    This product has been referenced in:
    • Shen L  et al. Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene. Antimicrob Agents Chemother 60:5492-503 (2016). Read more (PubMed: 27381385) »
    • Hashimoto S  et al. Mumps Virus Induces Protein-Kinase-R-Dependent Stress Granules, Partly Suppressing Type III Interferon Production. PLoS One 11:e0161793 (2016). Read more (PubMed: 27560627) »

    See all 7 Publications for this product

    レビューと Q&A

    Thank you for contacting us.

    The antibody is specific to phosphorylation site of T451, the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or info...

    Read More

    Der Antikörper sollte dies eigentlich unbeschadet überstanden haben. Wir versenden unsere Antikörper so z.B. auch nach Australien oder Thailand, und da kann es schon einmal zu Transitzeiten von einer Woche kommen, und bisher haben wir noch keine erhöht...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung epithelial)
    Loading amount
    10 µg
    Specification
    Lung epithelial
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    Verified customer

    投稿 Jul 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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