Anti-PKC zeta 抗体 (ab59364)
Key features and details
- Rabbit polyclonal to PKC zeta
- Suitable for: ELISA, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Xenopus laevis, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PKC zeta antibody
PKC zeta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC zeta -
由来種
Rabbit -
特異性
ab59364 detects endogenous levels of total PKC zeta protein. The immunogen used for this product shares 89% homology with PKC iota/lambda. Cross-reactivity with this protein has not been confirmed experimentally. -
アプリケーション
適用あり: ELISA, IHC-P, IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Xenopus laevis, African green monkey -
免疫原
Synthetic peptide within Human PKC zeta aa 526-575. The exact sequence is proprietary. Synthesized non-phosphopeptide derived from human PKC zeta around the phosphorylation site of threonine 560 (Q-L-TP-P-D).
Database link: Q05513 -
ポジティブ・コントロール
- IHC-P: Human proliferative endometrial tissue, human brain tissue. ICC: NIH/3T3 cells. WB: Rat hippocampus lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/50 - 1/100.
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IP |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/50 - 1/100. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
組織特異性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメイン
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻訳後修飾
CDH5 is required for its phosphorylation at Thr-410. -
細胞内局在
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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参照データベース
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 25522 Rat
- Omim: 176982 Human
- SwissProt: Q05513 Human
- SwissProt: Q02956 Mouse
- SwissProt: P09217 Rat
- Unigene: 496255 Human
see all -
別名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
画像
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Anti-PKC zeta antibody (ab59364) at 1/500 dilution + Xenopus laevis cytoplasmic egg extract at 40 µg
Secondary
Goat polyclonal - IRDye® 800CW at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 seconds
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Immunohistochemical analysis of human proliferative endometrial tissue, staining PKC zeta with ab59364.
Antigen retrieval was performed by heat mediation in a citrate buffer. Tissue was blocked with blocking solution before incubating with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB. -
Immunofluorescence analysis of NIH/3T3 cells using PKC zeta at dilution of 1:100.
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All lanes : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lane 1 : Extracts from COS-7 (African green monkey kidney fibroblast-like cell line) cells, treated with PMA (125 ng/ml, 30mins), without immunizing peptide
Lane 2 : Extracts from COS7 (African green monkey kidney fibroblast-like cell line)cells, treated with PMA (125 ng/ml, 30mins), plus immunizing peptide
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Lanes 2-4 : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lanes 1-2 : Rat hippocampus lysate at 40 µg
Lanes 3-4 : Rat hippocampus lysate at 20 µg
Secondary
All lanes : Anti-Rabbit IR800-linked conjugation: IRDye® 800CW
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 minutes
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ab59364 at a 1/50 dilution staining PKC zeta in formalin/PFA fixed paraffin-embedded human brain tissue using Immunohistochemistry.
Left panel: Without immunizing peptide.
Right image: Plus immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab59364 は 21 報の論文で使用されています。
- Ma R et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland. Cell Rep 38:110375 (2022). PubMed: 35172155
- Liu S et al. G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ. Front Genet 13:1034889 (2022). PubMed: 36330442
- Kasashima H et al. Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population. Dev Cell 56:95-110.e10 (2021). PubMed: 33207226
- Ryota H et al. Clinicopathological characteristics of pancreatic ductal adenocarcinoma with invasive micropapillary carcinoma component with emphasis on the usefulness of PKCζ immunostaining for detection of reverse polarity. Oncol Lett 22:525 (2021). PubMed: 34055090
- Ratnayake WS et al. Atypical PKCs activate Vimentin to facilitate prostate cancer cell motility and invasion. Cell Adh Migr 15:37-57 (2021). PubMed: 33525953