Application
Western blot
Sample
Cow Cell lysate - other (Cerebral microvascular endothelial cells)
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris)
Loading amount
10 µg
Specification
Cerebral microvascular endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 7.5% Milk in TBST
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: 926-68073 IRDye® 680RD Donkey anti-Rabbit IgG (H +
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Detection
Detection method
LiCor
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 105 kDa
Positive control
None
Additional data
Additional Notes
Lane shows primary cultured cerebral microvascular endothelial cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Nicholas Klug
Verified customer
投稿 Jun 26 2015