Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount
20000 cells
Specification
HT1080 Cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2.5% Milk + 0.05% Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 84 kDa Non-specific: 60, 55, 48, 45, 43, kDa
Additional data
Additional Notes
Lots of additional bands. It's possible that levels of PKC-gamma are low in these cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 10 2010